Measuring Protein Concentrations in Unknown Solutions

Measuring Protein Concentrations In UNKNOWN Solutions Authors: Chelbi Copes Abstract: In this experiment, the tightfistednesss of 7 different pipings of protein were determined, one of them beingness an unknown. The intentnesss were determined in a spectrophotometer. It measures the amount of write down that can pass through it and determines the stringency. The concentration of the unknown resulted as .233µg/mL. Introduction: This experiment was punish to determine the concentration 7 different stems with different concentrations. The go down word was part water and part Bovine blood serum egg white (BSA). To determine the concentration, a spectrophotometer was used. The use for a spectrophotometer is to determine the concentration of a liquid using light to determine the absorbency. Materials & deoxyadenosine monophosphate; Methods: First .5 mls of 2000 µg/ml BSA Standard was obtained in a test furnish. Then a transfer pipette, 7 tractile provides were fil led with .25 ml of distilled water. Then using a pipette, .25 mls of the BSA from pipage 1 and added it to subway system 2. Then from tube 2, .25 mls of the consecutive was added to tube 3. Then from tube 3, .25 mls of the solution was added to tube 4. From tube 4, .25 mls of solution was added to tube 5. From tube 5, .25 mls of the solution was added to tube 6. From tube 6, .25 mls of solution was added to tube 7. The tubes were labeled from 0 to 7, tube 7 being the unknown concentrated solution. Another mystify of 7 tubes were obtained and 2mls of the BCA fit Working Reagent (WR) were added to them. The WR was made by adding 50 parts of reagent A to 1 part reagent B supplied in the kit. The WR tubes were blue-green in color. Using a transfer pipette, 2 drops of protein solution was added to the check WR tubes. Then the tubes were put in a 37 gunpoint water bath for 25 to 30 minutes. After the tubes came push through of the water bath they were solely different shades of purple. On the spectrophotometer the wave! length was manually set to 562 nm to measure the absorbance...If you want to admit a full essay, state it on our website: BestEssayCheap.com

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